Primer Design of SNP rs4506565 Transcription Factor 7 like 2 (TCF7L2) Gene to Detect Type-2 Diabetes Mellitus

DOI

10.2991/ahsr.k.211105.029How to use a DOI?

Keywords

Primer; TCF7L2; SNP rs4506565; Type 2 Diabetes Mellitus

Abstract

Diabetes Mellitus is a chronic disease caused by a deficiency or insufficient insulin that increases the levels of blood glucose. Diabetes Mellitus causes several complications that can increase the numbers of mortality. SNP rs4506565 Transcription Factor 7 Like 2 (TCF7L2) gene reported to be strongly correlated with Type-2 Diabetes Mellitus (T2DM) in ethnic Caucasian, East Asian, and South Asian. SNP rs4506565 is indicated by polymorphism at point 112,996,282 from nucleotide Adenine (A) to Thymine (T). Amplification-Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) was used to detect the existence of polymorphism by its specific primer. The objective of this study was to construct a particular primer to identify the SNP rs4506565 TCF7L2 gene using ARMS PCR. This research was a descriptive study to figure the specific primer of the PCR reaction. The research process begins with isolating the DNA sample, followed by primer construction using Geneious software, doing PCR to amplify the specific area, and DNA sequencing. Based on the research, four primers have been designed to detect the SNP rs4506565 TCF7L2 gene. The primers were rs4506565-F, rs4506565-R, rs4506565-F(A) as a specific primer for the allele A, and rs4506565- R(T) to detect the allele T. The reaction produced three fragments of 650bp, 271bp, and 417bp. It can be concluded that the primer can be used in a small laboratory to detect the presence of rs4506565 TCF7L2 gene and identify the association between variant TCF7L2 gene SNP rs4506565 with T2DM as a preventive tool of T2DM in other ethnic.

Copyright

© 2021 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Elsa Badriyya
AU  - Afifatul Achyar
AU  - Syamsurizal
PY  - 2021
DA  - 2021/11/17
TI  - Primer Design of SNP rs4506565 Transcription Factor 7 like 2 (TCF7L2) Gene to Detect Type-2 Diabetes Mellitus
BT  - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021)
PB  - Atlantis Press
SP  - 197
EP  - 202
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.211105.029
DO  - 10.2991/ahsr.k.211105.029
ID  - Badriyya2021
ER  -