Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)

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Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)

Authors
Anifatus Sa’adah1, Muhtadi1, *
1Faculty of Pharmacy, Universitas Muhammadiyah Surakarta (UMS), Surakarta, Indonesia
*Corresponding author. Email: Muhtadi@ums.ac.id
Corresponding Author
Muhtadi
Available Online 14 December 2022.
DOI
10.2991/978-94-6463-050-3_15How to use a DOI?
Keywords
Protein; Albumin; α-glucosidase
Abstract

The giant gourami (Osphronemus goramy), rice eel (Monopterus albus), and mackerel tuna (Euthynnus affinis) are among the sources of protein and albumin that have been used to lower the blood glucose level of people with Diabetes Mellitus (DM). This study aims to determine the protein levels and albumin content of O. goramy, M. albus, and E. affinis and the inhibition activity of albumin against the α-glucosidase enzyme. This study employed an experimental method with accidental sampling. The protein content of fresh fish fillets was tested using the Kjeldahl method and extracted using a centrifuge to obtain albumin. Albumin levels were determined using a visible spectrophotometer. The inhibitory activity of albumin against α-glucosidase enzyme was tested using an ELISA reader. The results showed a significant difference between freshwater and seawater fish proteins, namely giant gourami of 13.91%, rice eel of 14.41%, and mackerel tuna of 30.55%. The highest albumin content was obtained from mackerel tuna (4.75 ± 0.04 g/100 mL), followed by giant gourami (3.61 ± 0.26 g/100 mL), and rice eel (2.38 ± 0.26 g/100 mL). The albumin showed no significant activity against the α-glucosidase enzyme.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
Series
Atlantis Highlights in Chemistry and Pharmaceutical Sciences
Publication Date
14 December 2022
ISBN
10.2991/978-94-6463-050-3_15
ISSN
2590-3195
DOI
10.2991/978-94-6463-050-3_15How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Anifatus Sa’adah
AU  - Muhtadi
PY  - 2022
DA  - 2022/12/14
TI  - Inhibitory Activity of the α-Glucosidase Enzyme by Albumin Isolated from Giant Gourami (Osphronemus Goramy), Rice Eel (Monopterus albus), and Mackerel Tuna (Euthynnus affinis)
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 179
EP  - 188
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_15
DO  - 10.2991/978-94-6463-050-3_15
ID  - Sa’adah2022
ER  -