Determination of Bergenin in Pulmonaria officinalis by High Performance Capillary Electrophoresis

DOI

10.2991/icammce-18.2018.12How to use a DOI?

Keywords

capillary electrophoresis, bergenin, Pulmonaria officinalis

Abstract

This paper presents the determination of bergenin content of Pulmonaria officinalis by high performance capillary electrophoresis (HPCE) method. The borax solution as buffer solution was chosen, and its concentration was 20 mmol/L at a constant voltage of 20kV and injecting time of 10s. Linearity was kept in the concent ration range of 0.085~1.35mg/mL of bergenin with correlation coefficient of 0.998. Measured bergenin content in Pulmonaria officinalis was 0.231 mg/g (RSD = 3.44%) (n = 6). The recovery was in the range of 97% - 116% (n=6). This method is specific, simple and rapid and accurate, which is suitable for the detection of the content of bergenin in Pulmonaria officinalis.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiao-Ri Sun
AU  - Xiao-Ping Zhang
AU  - Quan-Hua Fan
AU  - Hai-Xing Liu
PY  - 2018/05
DA  - 2018/05
TI  - Determination of Bergenin in Pulmonaria officinalis by High Performance Capillary Electrophoresis
BT  - Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)
PB  - Atlantis Press
SP  - 57
EP  - 61
SN  - 2352-5401
UR  - https://doi.org/10.2991/icammce-18.2018.12
DO  - 10.2991/icammce-18.2018.12
ID  - Sun2018/05
ER  -