Coating PAMAM dendrimer onto PDMS microfluidic chip to resist nonspecific Biomolecules adsorption
- 10.2991/icaemt-15.2015.41How to use a DOI?
- PAMAM dendrimer; microfluidic chip; caoting; hydrophilicity; adsorption
The surface of poly(dimethylsiloxane)(PDMS) microfluidic chips were modified using polyamidoamine (PAMAM) dendrimer via physical coating. The results showed that the surface of the modified chips was coated by a dense, uniform, continuous, hydrophilicity layer of PAMAM. The hydrophilic of the chip surface was markedly improved. The contact angle of the chips modified decreased from108.9° to 46.1°. Adenosine and L-lysine were separated via the modified PDMS microfluidic chips. Compared with unmodified chips, the modified chips successfully separated the two biomolecules. The separation efficiencies of adenosine and L-lysine were 7.19×104 Plates/m and 8.111×104 Plates/m respectively, and the resolutions(RS) was 4.03. This research may provide a new and effective method to resist nonspecific adsorptionon the PDMS surface and the application of PDMS microfluidic chips in the determination of trace biomolecules.
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Dong Lin AU - Qin Zhao AU - Miaomiao Yan AU - Giuge Hou PY - 2015/08 DA - 2015/08 TI - Coating PAMAM dendrimer onto PDMS microfluidic chip to resist nonspecific Biomolecules adsorption BT - Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology PB - Atlantis Press SP - 204 EP - 208 SN - 2352-5401 UR - https://doi.org/10.2991/icaemt-15.2015.41 DO - 10.2991/icaemt-15.2015.41 ID - Lin2015/08 ER -