Hardware Integration and Software Design of Automated Nucleic acid extraction System based on Magnetic Bead Separation Method

DOI

10.2991/ic3me-15.2015.269How to use a DOI?

Keywords

nucleic acid extraction; magnetic bead; Plant viral RNA

Abstract

This paper presents a system designed for automated nucleic acid extraction machine based on magnetic bead separation method. A hardware platform has been established which consists of motor controlling module, temperature controlling module and a computer; also, we designed the system software to achieve data acquisition, calculation, display, management and acid extraction control. The sample temperature achieved the required value within 13 min, and the accuracy of temperature control can be up to about ±1 degrees Celsius, when the sample amount is the maximum capacity of the sample matrix. And each nucleic acid purification experiment time is controlled within 60min. Plant viral RNA extraction experiments have been done on this platform. The automatic nucleic acid extraction instrument can be successfully used for the purification of DNA and RNA, and achieved very satisfactory experimental results.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yang Yu
AU  - Yongjie Dou
AU  - Qian Li
AU  - Xiaoli Zhao
AU  - Jiansheng Xu
AU  - Congliang Deng
AU  - Ming Wang
PY  - 2015/08
DA  - 2015/08
TI  - Hardware Integration and Software Design of Automated Nucleic acid extraction System based on Magnetic Bead Separation Method
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 1398
EP  - 1403
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.269
DO  - 10.2991/ic3me-15.2015.269
ID  - Yu2015/08
ER  -