Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering

Fast establishment and application of caspase-3 inhibitor detection system

Authors
Dan Wu, Hongye Dan, Huan Liu, Peng Yu, Yuou Teng
Corresponding Author
Dan Wu
Available Online August 2015.
DOI
https://doi.org/10.2991/ic3me-15.2015.109How to use a DOI?
Keywords
Recombinant human Caspase-3 . Expression . Purification. Ac-DEVE-CHO.
Abstract

Our objective to study the expression and activity of the apoptosis protease Caspase-3 in E. coli BL21 (DE3) Plyss. The cDNA of Caspase-3 was amplified by PCR and inserted into the cell of K562, it was then cloned to prokaryotic expression vector pET-21b, after which Caspase-3 was induced by IPTG. The Protein induced was identified by SDS-PAGE and Western blot. After induced by IPTG for 5 hours, the concentration of Caspase-3 reached the highest level. Finally active Caspase-3 can be induced within E. coli BL21 (DE3) Plyss, further research can be done to chose the Caspase-3 inhibitors.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
August 2015
ISBN
978-94-6252-100-1
ISSN
2352-5401
DOI
https://doi.org/10.2991/ic3me-15.2015.109How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Dan Wu
AU  - Hongye Dan
AU  - Huan Liu
AU  - Peng Yu
AU  - Yuou Teng
PY  - 2015/08
DA  - 2015/08
TI  - Fast establishment and application of caspase-3 inhibitor detection system
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 548
EP  - 551
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.109
DO  - https://doi.org/10.2991/ic3me-15.2015.109
ID  - Wu2015/08
ER  -