Investigation of Extraction and Purification Technology of Total Saponins from Sindora glabra Seeds

DOI

10.2991/ic3me-15.2015.94How to use a DOI?

Keywords

Sindora glabra seeds, Total saponins, Extraction process, Orthogonal experiments.

Abstract

Ginsenoside Re is employed as the reference substance for the determination of contents, elution rate of the total saponins from Sindora glabra seeds is as the evaluation index, the optimum extraction technology derives from the L9 (34) orthogonal experiments designed for purpose of the maximum extraction of total saponins. The results showed that the optimum extraction process is: concentration of ethanol 70%(v/v), temperature for extraction 50 , ratio of crude materials to liquid 1:15, time 2.5h, XAD resin is employed to extract the desired substrates total saponins, flow rate 1.0mL/min, desorption first by 6bv distilled water, followed by ethanol 7BV70%. The adsorption capacity of XAD macroporous resin on the total saponins is proved to be large and easy to be desorption.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yiping Chen
AU  - Chen Chen
AU  - Zhenyi Liang
AU  - Bo Wang
AU  - Pinghuai Liu
PY  - 2015/08
DA  - 2015/08
TI  - Investigation of Extraction and Purification Technology of Total Saponins from Sindora glabra Seeds
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 473
EP  - 476
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.94
DO  - 10.2991/ic3me-15.2015.94
ID  - Chen2015/08
ER  -