Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering

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Antioxidant activity of collagen hydrolysate obtained from tilapia scales in vitro and in vivio assays

Authors
Shaokui Zeng, Wenlong Wu, Chaohua Zhang, Juanjuan Yin, Zhenlin Li
Corresponding Author
Shaokui Zeng
Available Online August 2015.
DOI
10.2991/ic3me-15.2015.59How to use a DOI?
Keywords
Tilapia scale; collagen hydrolysate; antioxidant
Abstract

Collagen hydrolysate of S and C were prepared by enzymatic hydrolysis decalcified tilapia scale and pepsin-solubilised collagen extracted from decalcified tilapia scale. The antioxidative activity of C and S were evaluated by their effects on scavenging ability of superoxide anion O2- radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reductive ability in vivo. Besides, the activity of glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and content of malondialdehyde (MDA) in vitro of the laboratory mouse were detected. The results indicated that collagen hydrolysate of C and S show prominent inhibitory effect on scavenging O2- with the fifty-percent inhibitory concentration (IC50) of 3.7 and 6.5 mg/mL, respectively. The IC50 of collagen hydrolysate of C and S inhibit the DPPH were 8.4 and 7.2 mg/mL, respectively. Two collagen hydrolysates demonstrated highly reducing activity. In vivo experimentation suggested that C and S can significantly improve the GSH-Px activity, T-AOC and reduce the contents of MDA. As a consequence of results, collagen hydrolysates of C and S extracted from decalcified tilapia scale have a good antioxidation.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
August 2015
ISBN
10.2991/ic3me-15.2015.59
ISSN
2352-5401
DOI
10.2991/ic3me-15.2015.59How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Shaokui Zeng
AU  - Wenlong Wu
AU  - Chaohua Zhang
AU  - Juanjuan Yin
AU  - Zhenlin Li
PY  - 2015/08
DA  - 2015/08
TI  - Antioxidant activity of collagen hydrolysate obtained from tilapia scales in vitro and in vivio assays
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 311
EP  - 315
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.59
DO  - 10.2991/ic3me-15.2015.59
ID  - Zeng2015/08
ER  -