Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering

Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP

Authors
Lingzhi Zhao, Liu Zhao, Yanqing Miao, Chunye Liu
Corresponding Author
Lingzhi Zhao
Available Online August 2015.
DOI
https://doi.org/10.2991/ic3me-15.2015.27How to use a DOI?
Keywords
colorimetric; cysteine; reduced glutathione; homocysteine; Gold nanoparticles (AuNPs)
Abstract

Based on the dependence between the AuNPs size and the naked-eye visible color change. This study was designed to develop a highly selective and sensitive towards colorimetric sensing of cysteine in fetal bovine serum by the competitive binding of ATP and thiol compounds (cysteine and homocysteine) towards gold nanoparticles (AuNPs). The as-established method has high sensitivity and selectivity, the linearity range was 3 M-30 M with the quantification limit of 1 M. The method demonstrated here can be applied to detect cysteine in fetal bovine serum without interference from other amino acids and bovine serum albumin (BSA), which is envisaged to be applicable to understanding the chemical essence involved in physiological and pathological events associated with cysteine.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
August 2015
ISBN
978-94-6252-100-1
ISSN
2352-5401
DOI
https://doi.org/10.2991/ic3me-15.2015.27How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Lingzhi Zhao
AU  - Liu Zhao
AU  - Yanqing Miao
AU  - Chunye Liu
PY  - 2015/08
DA  - 2015/08
TI  - Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 141
EP  - 146
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.27
DO  - https://doi.org/10.2991/ic3me-15.2015.27
ID  - Zhao2015/08
ER  -