Immunomodulator Activity of 5,7-dihydroxy isoflavones and β-Sitosterol from Peronema canescens Jack Leaves Methanol and Ethyl Acetate Extract

DOI

10.2991/978-2-38476-110-4_57How to use a DOI?

Keywords

Bioactive compound; Immunomodulator; P. canescens

Abstract

The end of 2019 marked the beginning of a global pandemic with the emergence of the Corona Virus or Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) which attacks the respiratory system and causes this viral infectious disease called COVID-19. A good immune system is needed to support health and endurance in pandemic condition. One of the plants that have the potential to be developed as an immunomodulator is the Sungkai plant (Peronema canescens Jack). The aim of this study was to extract the bioactive compounds of P. canescens Jack leaves and its immunomodulatory activity against white male mice. P. canescens Jack was extracted using Ethyl acetate and methanol, followed by the isolation process using Liquid Vacuum Chromatography (LVC) to obtain single compound (EI) from the ethyl acetate fraction and single compound (MI) from the methanol fraction. Immunomodulator activity was tested using mice, using doses of 50 mg/Kgbb, 100 mg/Kgbb, 150 mg/Kgbb, and 450 mg/Kgbb. The methanol extract of 450 mg/KgBb P. canescens Jack leaves had the largest number of active leukocytes and macrophages with a value of 64.9%. Meanwhile, at a dose of 450 mg/KgBW EI, there was an increase in leukocytes and the highest % activity of active macrophages which was almost close to the positive control (C+). Based on the comparison of the literature, the UV-Vis and FTIR spectrum patterns of isolate F3 are β-Sitosterol compounds, while F2 is a 5.7 dihydroxy isoflavone compound. Our finding that two compounds of P. cannescens Jack leaves extract β-Sitosterol and 5,7-dihydroxy isoflavone which have immunomodulatory activity.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Madyawati Latief
AU  - Sutrisno
AU  - Ellyn Dasrinal
AU  - Wulan Safitri
AU  - Indra Lesmana Tarigan
PY  - 2023
DA  - 2023/11/07
TI  - Immunomodulator Activity of 5,7-dihydroxy isoflavones and β-Sitosterol from Peronema canescens Jack Leaves Methanol and Ethyl Acetate Extract
BT  - Proceedings of the 4th Green Development International Conference (GDIC 2022)
PB  - Atlantis Press
SP  - 558
EP  - 572
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-110-4_57
DO  - 10.2991/978-2-38476-110-4_57
ID  - Latief2023
ER  -