Identification and enzymatic activities of psychrophilic yeasts isolated from permafrost soil in Mongolia

DOI

10.2991/978-94-6463-278-1_9How to use a DOI?

Keywords

Cystofilobasium ssp.; enzyme activity; Naganishia ssp.; psychrophilic-psychrotolerant yeasts; Vishniacozyma ssp.

Abstract

Psychrophilic yeasts with unique properties have recently become a new source of biologically active products. However, psychrophilic microorganisms, including yeasts that inhabit Mongolian permafrost, are practically unknown. Therefore, this study was the first attempt to reveal living organisms hidden in Mongolian permafrost, in particular yeasts, by isolating and identifying yeast strains, investigating their physiological characteristics and the ability to produce extracellular enzymes. Fifteen strains were isolated at 0°C from soil samples collected from three different permafrost sites in Mongolia. The strains were identified by the D1 / D2 domain sequences of the LSU rRNA gene, and as a result, 6 strains belong to the genus Cystofilobasidium, 7 and 2 strains belong to the genera Naganishia and Vishniacozyma, respectively. Testing the effect of temperature on the growth of the 15 strains showed that all of them were able to grow at 0, 4 and 15°C, 6 strains were able to grow at 28°C, and none of them were able to grow at 37°C. In addition, the extracellular enzyme activity of all strains was determined, 10 strains exhibited lipolytic activity and 11 strains cellulolytic activity, respectively. These yeasts exhibited extracellular enzymatic activity at 0, 4, 15, and 28°C, indicating that they maintain active metabolism under permafrost conditions, which is of great importance both for studying cold-adapted enzymes and microbial activity in the degradation of the permafrost habitat.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Enkh-Amgalan Jigjiddorj
AU  - Bumtsend Baymbasuren
AU  - Enkh-Undral Battsengel
AU  - Sarantuya Jargalsaikhan
PY  - 2023
DA  - 2023/10/29
TI  - Identification and enzymatic activities of psychrophilic yeasts isolated from permafrost soil in Mongolia
BT  - Proceedings of the Fourth International Conference on Environmental Science and Technology (EST 2023)
PB  - Atlantis Press
SP  - 76
EP  - 88
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-278-1_9
DO  - 10.2991/978-94-6463-278-1_9
ID  - Jigjiddorj2023
ER  -