Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics

An Optimized Preparation Method to Obtain High-quality RNA from the Buds of Sunflowers

Authors
Hao Feng, XiBao Ma, Jun Yang
Corresponding Author
Hao Feng
Available Online August 2016.
DOI
10.2991/emcpe-16.2016.18How to use a DOI?
Keywords
Sunflower Buds; RNA Extraction; Polyphenol; Polysaccharide
Abstract

In an attempt to isolate high-quality, total RNA from flower buds of sunflowers (Helianthus annuus). We have already improved the SDS method. After adding liquid nitrogen, we have to grind the material down. After that we add protein -DNA precipitation, wash it with the addition of different kinds of alcohol, such as 75% alcohol and precipitation. Then we add DEPC water and guanidine hydrochloride blend, LiCl overnight precipitation,and then wash it off with 75% alcohol, finally make it dissolved in 15 æL DEPC water which collocated in minus 80 environment for storage. The total RNA is precipitated with 1/10 volume of NaAc and 2 volumes of absolute ethanol to prevent contamination by polysaccharides. These experimental results show that the total RNA extracted by this method is of high purity and integrity, and the electrophoretic bands are clear. D260/D230, D260/D280 and RNA (g/100mg) are 2.05, 2.11 and 9.52, respectively. Total RNA can amplify the target gene fragment by PT-PCR. This improved method can be used to extract the high-quality RNA from the sunflower buds of the space mutation, in order to study the molecular mechanism of the next step.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics
Series
Advances in Engineering Research
Publication Date
August 2016
ISBN
978-94-6252-197-1
ISSN
2352-5401
DOI
10.2991/emcpe-16.2016.18How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hao Feng
AU  - XiBao Ma
AU  - Jun Yang
PY  - 2016/08
DA  - 2016/08
TI  - An Optimized Preparation Method to Obtain High-quality RNA from the Buds of Sunflowers
BT  - Proceedings of the 2016 5th International Conference on Environment, Materials, Chemistry and Power Electronics
PB  - Atlantis Press
SP  - 78
EP  - 85
SN  - 2352-5401
UR  - https://doi.org/10.2991/emcpe-16.2016.18
DO  - 10.2991/emcpe-16.2016.18
ID  - Feng2016/08
ER  -