A Preliminary Study on DNA Damage in Peripheral Blood of Cancer Patients After Radiotherapy with Comet Assay

DOI

10.2991/ahsr.k.200523.056How to use a DOI?

Keywords

DNA damage, cancer, radioteraphy, comet assays

Abstract

The aim of this study was to look at DNA damage studied in cancer patients during radiotherapy using the comet test. Subjects were from patients diagnosed with different solid tumors who received radiotherapy and control groups adjusted for age, sex. Blood samples were collected from patients with several types of cancer after receiving cumulative radiation doses of 10, 12 and 14 Gy. The level of DNA damage was evaluated using the alkaline comet test on peripheral blood lymphocytes. Samples consisting of 3 ml of whole blood were collected by venipuncture. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and The frequency of DNA cells was evaluated by calculating a total of 50 cells per slide. data analysis was done using CaspLab program. The comet test results showed that the average tail length, tail DNA, and tail moments of radiotherapy patients were higher than the control group and significantly different between both group (P <0.01). There is a positive correlation between radiation doses received by patient with DNA damage. The conclusion is that exposure to ionizing radiation leads to an increase in the level of DNA damage in the patient’s peripheral blood lymphocytes. Meanwhile, it was found that there was inter-individual variability in response to radiotherapy among patients.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Darlina Yusuf
AU  - Tur Rahardjo
AU  - Viria Agesti Suvivan
PY  - 2020
DA  - 2020/06/08
TI  - A Preliminary Study on DNA Damage in Peripheral Blood of Cancer Patients After Radiotherapy with Comet Assay
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 228
EP  - 232
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.056
DO  - 10.2991/ahsr.k.200523.056
ID  - Yusuf2020
ER  -