In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen

DOI

10.2991/ahsr.k.200523.025How to use a DOI?

Keywords

dengue virus, antigen, antibody, molecular docking

Abstract

Objectives: The aim of this study was to determine the interaction between Indonesian NS1 DENV-2 with 6D4B10 and 8A6F2 single chain fragment variable antibodies. Method: In this study, bioinformatics approach was performed on the six structures of Indonesian NS1 antigenes of DENV-2 to obtain the best model using BLAST, Pyre2, Modeller 9.20 and the FireDock server for molecular docking. Results and Discussion: We obtained the binding energy of 6D4B10 antibody to NS1 D2/ID/SMG-SE001 was -55.04 kcal/mol and 8A6F2 antibody to D2/ID/JKT-J002 was -49.42 kcal/mol. The interactions of antigen-antibody complex were hydrogen bonds, electrostatic interactions, hydrophobic interactions and several other interactions. Conclusion: The results showed that 6D4B10 and 8A6F2 antibodies can be provide for designing molecular antibody fragments as one of dengue diagnostic kits agent in Indonesia.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dewi Astriany
AU  - Yeni Indah Puspita Sari
AU  - Umi Baroroh
AU  - Muhammad Yusuf
AU  - Iman Permana Maksum
AU  - Desy Natalia
AU  - Toto Subroto
PY  - 2020
DA  - 2020/06/08
TI  - In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 102
EP  - 106
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.025
DO  - 10.2991/ahsr.k.200523.025
ID  - Astriany2020
ER  -