In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen
- DOI
- 10.2991/ahsr.k.200523.025How to use a DOI?
- Keywords
- dengue virus, antigen, antibody, molecular docking
- Abstract
Objectives: The aim of this study was to determine the interaction between Indonesian NS1 DENV-2 with 6D4B10 and 8A6F2 single chain fragment variable antibodies. Method: In this study, bioinformatics approach was performed on the six structures of Indonesian NS1 antigenes of DENV-2 to obtain the best model using BLAST, Pyre2, Modeller 9.20 and the FireDock server for molecular docking. Results and Discussion: We obtained the binding energy of 6D4B10 antibody to NS1 D2/ID/SMG-SE001 was -55.04 kcal/mol and 8A6F2 antibody to D2/ID/JKT-J002 was -49.42 kcal/mol. The interactions of antigen-antibody complex were hydrogen bonds, electrostatic interactions, hydrophobic interactions and several other interactions. Conclusion: The results showed that 6D4B10 and 8A6F2 antibodies can be provide for designing molecular antibody fragments as one of dengue diagnostic kits agent in Indonesia.
- Copyright
- © 2020, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Dewi Astriany AU - Yeni Indah Puspita Sari AU - Umi Baroroh AU - Muhammad Yusuf AU - Iman Permana Maksum AU - Desy Natalia AU - Toto Subroto PY - 2020 DA - 2020/06/08 TI - In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen BT - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019) PB - Atlantis Press SP - 102 EP - 106 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.200523.025 DO - 10.2991/ahsr.k.200523.025 ID - Astriany2020 ER -