Proceedings of the 4th Borobudur International Symposium on Science and Technology 2022 (BIS-STE 2022)

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Influence of Extraction Solvents on Capsaicin Compound and Cytotoxic Activity of Piper retrofractum Vahl

Authors
Kirana Anggraini1, *, Dyah Ayu Woro Setyaningrum1, Laela Wulansari1, 2, Hening Tyas Andayani3, Laviany Putri Shihran1, Isra Fauziyyah1
1Universitas Trisakti, Jakarta, Indonesia
2Tropical Biopharmaca Research Center, Institute for Research and Community Service (LPPM), IPB University, Bogor, Indonesia
3Fachärztin der Innere Medizin, Städtische Kliniken, Mönchengladbach, Germany
*Corresponding author. Email: kirana_anggraeni@trisakti.ac.id
Corresponding Author
Kirana Anggraini
Available Online 9 November 2023.
DOI
10.2991/978-94-6463-284-2_89How to use a DOI?
Keywords
Extraction Solvents; Capsaicin Compound; Cytotoxic Activity; Piper retrofractum Vahl
Abstract

Capsaicin (8-Methyl-N-vanillyl-trans-6-nonenamide) is an active compound found in plants from the genus Capsicum. Genus capsium posseses different species of red chilli (Capsicum annuum Linn), green chilli (Capsicum annuum var.annuum) and other varieties that are familiar in Indonesia. However, the plants were not originally from Indonesia but from American Continent. Indonesian ancestor utilized chilli to add spiciness on foods which refers to Piper retrofractumVahl or Javanese chilli. Capsaicin has positive effects on health such as analgesic, cardioprotective, anorexigenic, chemopreventive, chemotherapy, and cardioprotective effects. In addition, capsaicin has beneficial effects in maintaining glucose levels, insulin homeostasis, reducing itching and symptoms in Non-Allergic Rhinitis (NAR), and an alternative therapy for neurogenic bladder. This research is aimed to acknowledge if capsaicin is also discovered in Javanese chilli. The method used to obtain the extract was by maceration of Java chilies using 30%, 70%, and 96% ethanol, n-hexane, ethyl acetate, and water as solvents. The research was started by conducting phytochemical and moisture content tests on simplicia. Then, each extract was tested for cytotoxic activity on shrimp larvae and the determination of capsaicin levels by High Performance Liquid Chromatography (HPLC). The results showed that the highest yield of Java chili extract was 51.14% in ethanol 70%. Phytochemical testing of Java chili simplicia contains flavonoids and saponins. The ethanol 70% extract had the highest cytotoxic activity with an LC50 value of 205.21 ppm. The highest concentration of capsaicin was found in ethyl acetate solvent of 785.58 mg/g followed by ethanol 70% at 486.06mg/g.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th Borobudur International Symposium on Science and Technology 2022 (BIS-STE 2022)
Series
Advances in Engineering Research
Publication Date
9 November 2023
ISBN
978-94-6463-284-2
ISSN
2352-5401
DOI
10.2991/978-94-6463-284-2_89How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Kirana Anggraini
AU  - Dyah Ayu Woro Setyaningrum
AU  - Laela Wulansari
AU  - Hening Tyas Andayani
AU  - Laviany Putri Shihran
AU  - Isra Fauziyyah
PY  - 2023
DA  - 2023/11/09
TI  - Influence of Extraction Solvents on Capsaicin Compound and Cytotoxic Activity of Piper retrofractum Vahl
BT  - Proceedings of the 4th Borobudur International Symposium on Science and Technology 2022 (BIS-STE 2022)
PB  - Atlantis Press
SP  - 851
EP  - 859
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-284-2_89
DO  - 10.2991/978-94-6463-284-2_89
ID  - Anggraini2023
ER  -