Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

Spectrophotometric Determination of Penicillamine in Pharmaceutical Sample using Fe(III)-Tiron System

Authors
Xinrong WEN, Changqing TU
Corresponding Author
Xinrong WEN
Available Online December 2016.
DOI
10.2991/bep-16.2017.33How to use a DOI?
Keywords
penicillamine;Fe(III); tiron; discoloration spectrophotometry
Abstract

Hydrosulfuryl(-SH) in penicillamine molecule can reduce Fe(III) to Fe(II), then using tiron as chromogenic reagent of Fe(III), and the content of penicillamine is determinated indirectly through determinating the surplus content of Fe(III) in the system. An accurate fast spectrophotometric method for the determination of penicillamine by discoloration spectrophotometry using Fe(III)-tiron system has been established. The various effect factors on the determination of penicillamine using Fe(III)-tiron system have been investigated in detail. The results show that when the reaction temperature was 85°C ,the reaction time was 20 min, the dosage of pH3.0 buffer solution was 15.00mL, the dosage of Fe(III) was 2.30mL, the dosage of tiron was 1.00mL, the maximum absorption wavelength of the complex of Fe(III)-tiron was 660 nm, good linear relationship was obtained between ΔA and the concentration of penicillamine in the range of 0.008000~0.04800 mg⋅mL-1,the equation of the linear regression was ΔA=0.0074+7.575C(mg⋅mL-1), with a linear correlation coefficient was 0.9993. This proposed method had been successfully applied to determinate of penicillamine in real pharmaceutical.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
Series
Advances in Biological Sciences Research
Publication Date
December 2016
ISBN
978-94-6252-287-9
ISSN
2468-5747
DOI
10.2991/bep-16.2017.33How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Xinrong WEN
AU  - Changqing TU
PY  - 2016/12
DA  - 2016/12
TI  - Spectrophotometric Determination of Penicillamine in Pharmaceutical Sample using Fe(III)-Tiron System
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 159
EP  - 162
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.33
DO  - 10.2991/bep-16.2017.33
ID  - WEN2016/12
ER  -