Spectrophotometric Determination of Penicillamine in Pharmaceutical Sample using Fe(III)-Tiron System
- DOI
- 10.2991/bep-16.2017.33How to use a DOI?
- Keywords
- penicillamine;Fe(III); tiron; discoloration spectrophotometry
- Abstract
Hydrosulfuryl(-SH) in penicillamine molecule can reduce Fe(III) to Fe(II), then using tiron as chromogenic reagent of Fe(III), and the content of penicillamine is determinated indirectly through determinating the surplus content of Fe(III) in the system. An accurate fast spectrophotometric method for the determination of penicillamine by discoloration spectrophotometry using Fe(III)-tiron system has been established. The various effect factors on the determination of penicillamine using Fe(III)-tiron system have been investigated in detail. The results show that when the reaction temperature was 85°C ,the reaction time was 20 min, the dosage of pH3.0 buffer solution was 15.00mL, the dosage of Fe(III) was 2.30mL, the dosage of tiron was 1.00mL, the maximum absorption wavelength of the complex of Fe(III)-tiron was 660 nm, good linear relationship was obtained between ΔA and the concentration of penicillamine in the range of 0.008000~0.04800 mg⋅mL-1,the equation of the linear regression was ΔA=0.0074+7.575C(mg⋅mL-1), with a linear correlation coefficient was 0.9993. This proposed method had been successfully applied to determinate of penicillamine in real pharmaceutical.
- Copyright
- © 2017, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xinrong WEN AU - Changqing TU PY - 2016/12 DA - 2016/12 TI - Spectrophotometric Determination of Penicillamine in Pharmaceutical Sample using Fe(III)-Tiron System BT - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016) PB - Atlantis Press SP - 159 EP - 162 SN - 2468-5747 UR - https://doi.org/10.2991/bep-16.2017.33 DO - 10.2991/bep-16.2017.33 ID - WEN2016/12 ER -