Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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Preliminary Study on The RT-LAMP Assay for Rabbit Hemorrhagic Disease Virus Type 2 Detection

Authors
Zexiao YANG, Yilin LUO, Zhengqun MENG, Xueping YAO, Han ZHANG, Yin WANG, Xiaoxue XIANG, Lijun ZHOU, Yadong LIU, Yan LI
Corresponding Author
Zexiao YANG
Available Online December 2016.
DOI
10.2991/bep-16.2017.27How to use a DOI?
Keywords
rabbit hemorrhagic disease; RHDV2; RT-LAMP; PCR; detection
Abstract

To develop a rapid molecular biological method for the detection of rabbit hemorrhagic disease virus type 2 (RHDV2), a set of 4 primers were designed according to the conserved sequence fragment of the capsid protein(VP60) gene of RHDV2 published in GenBank, and the reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was established through identification of target gene fragments, optimization of reaction conditions, sensitivity and specificity tests, and the application tests of 30 samples.The results showed the RT-LAMP method for the RHDV2 detection had a ladder-like pattern of amplification bands from about 119 bp incubation at 63.5°C for 90 min by using agarose gel electrophoresis, the sensitivity of the RT-LAMP could reach about 180 copies/µL of target fragments, there was no amplification for RHDV, pGM-T-EBHSV, Pasteurella multocida, E.coli and Salmonella from rabbits detection by this approach. The application tests results showed that there were not RHDV2 in the experimentally infected samples and clinical samples. The RT-LAMP assay established here had good specificity and sensitivity, which is suitable for RHDV2 rapid detection.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
Series
Advances in Biological Sciences Research
Publication Date
December 2016
ISBN
978-94-6252-287-9
ISSN
2468-5747
DOI
10.2991/bep-16.2017.27How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Zexiao YANG
AU  - Yilin LUO
AU  - Zhengqun MENG
AU  - Xueping YAO
AU  - Han ZHANG
AU  - Yin WANG
AU  - Xiaoxue XIANG
AU  - Lijun ZHOU
AU  - Yadong LIU
AU  - Yan LI
PY  - 2016/12
DA  - 2016/12
TI  - Preliminary Study on The RT-LAMP Assay for Rabbit Hemorrhagic Disease Virus Type 2 Detection
BT  - Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)
PB  - Atlantis Press
SP  - 127
EP  - 132
SN  - 2468-5747
UR  - https://doi.org/10.2991/bep-16.2017.27
DO  - 10.2991/bep-16.2017.27
ID  - YANG2016/12
ER  -