Proceedings of the 2016 International Conference on Biomedical and Biological Engineering

Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche

Authors
Xue Han, Bing Han
Corresponding Author
Xue Han
Available Online July 2016.
DOI
https://doi.org/10.2991/bbe-16.2016.68How to use a DOI?
Keywords
Transformation, Leaf Age, Scanning Electron Microscopy (SEM), Agrobacterium Attachment.
Abstract
Poplar as a woody-plant model is significant to improve the transformation efficiency of poplar. The effect of different leaf age as explants on transformation frequency was studied. The results exhibited that the higher transformation frequency was generated by 30-day-old leaf explants, compared with 60-day-old and 90-day-old leaf explants. The transformation frequency was more than 30%. The results indicated that various of transformation frequency were led by different leaf ages explants under the same conditions. The status of Agrobacterium attachment were observed by scanning electron microscopy (SEM) in the process of transformation. The results exhibited that Agrobacterium attachment were gradually reduced with the increased in leaf ages. Leaf surface at thirty-day-old showed massive Agrobacterium attachment. Our results suggest that younger leaves had higher transformation efficiency than older leaves.
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This is an open access article distributed under the CC BY-NC license.

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Proceedings
International Conference on Biomedical and Biological Engineering
Part of series
Advances in Biological Sciences Research
Publication Date
July 2016
ISBN
978-94-6252-216-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bbe-16.2016.68How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Xue Han
AU  - Bing Han
PY  - 2016/07
DA  - 2016/07
TI  - Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche
BT  - International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.68
DO  - https://doi.org/10.2991/bbe-16.2016.68
ID  - Han2016/07
ER  -