Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche

DOI

10.2991/bbe-16.2016.68How to use a DOI?

Keywords

Transformation, Leaf Age, Scanning Electron Microscopy (SEM), Agrobacterium Attachment.

Abstract

Poplar as a woody-plant model is significant to improve the transformation efficiency of poplar. The effect of different leaf age as explants on transformation frequency was studied. The results exhibited that the higher transformation frequency was generated by 30-day-old leaf explants, compared with 60-day-old and 90-day-old leaf explants. The transformation frequency was more than 30%. The results indicated that various of transformation frequency were led by different leaf ages explants under the same conditions. The status of Agrobacterium attachment were observed by scanning electron microscopy (SEM) in the process of transformation. The results exhibited that Agrobacterium attachment were gradually reduced with the increased in leaf ages. Leaf surface at thirty-day-old showed massive Agrobacterium attachment. Our results suggest that younger leaves had higher transformation efficiency than older leaves.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xue Han
AU  - Bing Han
PY  - 2016/07
DA  - 2016/07
TI  - Leaf Age Promotes Agrobacterium-mediated Transformation of Hybrid Poplar Populus Davidiana Dode × P. bollena Lauche
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 442
EP  - 446
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.68
DO  - 10.2991/bbe-16.2016.68
ID  - Han2016/07
ER  -