Fasudil Protects Against the Myocardial Hypertrophy of Transverse Aortic Constriction Via Modulating the Expression of Caspase-3 and Bcl-2 Protein

DOI

10.2991/bbe-16.2016.47How to use a DOI?

Keywords

Fasudil, Expression of Caspase-3 and Bcl-2 Protein, Cardiac Hypertrophy, Transverse, Aortic Constriction.

Abstract

Objectives: To investigate the effects of fasudil (Fas) on myocardial hypertrophy in rats. Methods Rats were randomly divided into 5 groups: control , transverse aortic constriction (TAC) model, Fas (5mg/kg) plus TAC, TAC plus Fas(20mg/kg), and TAC plus captopril (Cap, positive drug). After administering treatments for eight weeks, myocardial intracellular Ca2 + ion, cardiac hemodynamic parameters and histological changes, as well as NO and SOD levels of serum were measured. Protein expression changes in Caspase-3 and Bcl-2 were detected. Results Compared with the control group, rats in the TAC group showed increases in cardiac and left ventricular weight indexes, and had larger myocardial fiber diameters, as well as an increased incidence of myocardial fibrosis. Treatment of TAC-induced rats with Fas and Cap reduced the degree of these changes, improving myocardial contraction and relaxation functions, and significantly (p<0.05) reducing the free Ca2+ ion concentration of myocytes. The results indicated that Fas or Cap treatment can alleviate myocytes apoptosis. Furthermore, increasing expression of Caspase-3 and decreasing expression of Bcl-2 were observed in the TAC model group, and these changes could be alleviated by treatment with Fas and Cap. Conclusions Fas treatment significantly improved cardiac hypertrophy, which might be related to modulating the expression of Caspase-3 and Bcl-2 protein, enhancement of NO release of myocytes in rats after TAC

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Le Li
AU  - Hao Shang
AU  - Cailing Zhang
AU  - Houquan Tao
AU  - Jirkun Cheng
PY  - 2016/07
DA  - 2016/07
TI  - Fasudil Protects Against the Myocardial Hypertrophy of Transverse Aortic Constriction Via Modulating the Expression of Caspase-3 and Bcl-2 Protein
BT  - Proceedings of the 2016 International Conference on Biomedical and Biological Engineering
PB  - Atlantis Press
SP  - 297
EP  - 309
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbe-16.2016.47
DO  - 10.2991/bbe-16.2016.47
ID  - Li2016/07
ER  -