Sequence Analysis of the Gene CYP79B3 in Cabbage (Brassica Oleracea Var. Capitata)

DOI

10.2991/bbbs-18.2018.9How to use a DOI?

Keywords

Cabbage, glucosinolate, CYP79B3, sequence analysis.

Abstract

CYP79B3 is an important cytochrome P450 monooxygenases in glucosinolate biosynthesis. Here, the Brassica oleracea var. capitata CYP79B3 (BocCYP79B3) gene sequence was obtained from Brassica database (BRAD), and preformed for sequence analysis. The BocCYP79B3 gene mapped to Scaffold000053, and contains an open reading frame of 1,632 bp that encodes a 543-amino acid protein with a calculated molecular mass of 61.54 kD and an isoelectric point (pI) of 8.59. Subcellular localization predicted the BocCYP79B3 gene was in the chloroplast. The conserved domain of the BocCYP79B3 protein is PLN02971. The CYP79B3 protein is most closely related to B. napus. The findings of the present study provide a molecular basis for the elucidation of CYP79B3 gene function in cabbage.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Bo Sun
AU  - Min Jiang
AU  - Qiao Yuan
AU  - Fen Zhang
AU  - Haoru Tang
PY  - 2018/04
DA  - 2018/04
TI  - Sequence Analysis of the Gene CYP79B3 in Cabbage (Brassica Oleracea Var. Capitata)
BT  - Proceedings of the 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018)
PB  - Atlantis Press
SP  - 38
EP  - 41
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbbs-18.2018.9
DO  - 10.2991/bbbs-18.2018.9
ID  - Sun2018/04
ER  -