Expression and Sequence Analysis of HTH in Blackberry (Rubus Spp.)
Xunju Liu, Min Zhou, Yan Wang, Haoru Tang, Qing Chen
Available Online April 2018.
- 10.2991/bbbs-18.2018.1How to use a DOI?
- Rubus spp, HTH, Cloning and expression, Light irradiation.
To better understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene HTH was cloned from blackberry. The open reading frame of this gene was 1785 bp, encoding 594 amino acid residues with a molecular weight of 64.6 kD and a theoretical isoelectric point of 9.39. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the expression level of HTH gene decrease rapidly with the maturity of the fruit. The expression level of HTH decreased with the increase of red or blue light intensity. The lowest expression of HTH gene was under pure blue light treatment.
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xunju Liu AU - Min Zhou AU - Yan Wang AU - Haoru Tang AU - Qing Chen PY - 2018/04 DA - 2018/04 TI - Expression and Sequence Analysis of HTH in Blackberry (Rubus Spp.) BT - Proceedings of the 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018) PB - Atlantis Press SP - 1 EP - 5 SN - 2468-5747 UR - https://doi.org/10.2991/bbbs-18.2018.1 DO - 10.2991/bbbs-18.2018.1 ID - Liu2018/04 ER -