Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)

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The Effect of pH on the Activity of Bacteriocin from Lactobacillus acidophilus on the Growth of Salmonella typhi

Authors
Siska Telly Pratiwi, Alexander Siagian, Elizabeth Noviana Lumban Raja
Corresponding Author
Siska Telly Pratiwi
Available Online 24 July 2021.
DOI
10.2991/ahsr.k.210723.001How to use a DOI?
Keywords
Bacteriocin, Lactobacillus acidophilus, Salmonella typhi
Abstract

Typhoid fever is one of the most serious infectious diseases in Indonesia. Antibiotic resistance is one of the problems associated with the treatment of typhoid fever. Bacteriocin is an antimicrobial substance produced by L. acidophilus. Bacteriocin has bactericidal and bacteriostatic activity. The aim of this study was to determine the activity of bacteriocin from L. acidophilus against Salmonella typhi as antimicroba. The design of this study was laboratory experimental with posttest only control group design using the Kirby-Bauer agar diffusion method on Mueller Hinton Agar (MHA). The bacteria of this study were S. typhi ATCC 6539 and bacteriocin from L. acidophilus ATCC 4356 in various pH (2, 4, 6, 8, 10, and 12). The result of observation was carried out by measuring the inhibition zone in each treatment. The result showed bacteriocin from L. acidophilus at pH 2 had an inhibition zone 4.91 ±1.70 mm. Statistical calculation using the One Way Anova test showed that p-value was 0.000 then continued with the Post Hoc Tukey test showed bacteriocin from L. acidophilus at pH 2 inhibited the growth of S. typhi very significant compared to chloramphenicol. L. acidophilus can inhibit the growth of S. typhi and has an optimal pH, which is at pH 2 with a weak category of inhibitory strength. The bacteriocin from L. acidophilus ATCC 4356 could not compete with chloramphenicol as the first line drug for typhoid fever.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
Series
Advances in Health Sciences Research
Publication Date
24 July 2021
ISBN
978-94-6239-408-7
ISSN
2468-5739
DOI
10.2991/ahsr.k.210723.001How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Siska Telly Pratiwi
AU  - Alexander Siagian
AU  - Elizabeth Noviana Lumban Raja
PY  - 2021
DA  - 2021/07/24
TI  - The Effect of pH on the Activity of Bacteriocin from Lactobacillus acidophilus on the Growth of Salmonella typhi
BT  - Proceedings of the 12th Annual Scientific Meeting, Medical Faculty, Universitas Jenderal Achmad Yani, International Symposium on "Emergency Preparedness and Disaster Response during COVID 19 Pandemic" (ASMC 2021)
PB  - Atlantis Press
SP  - 1
EP  - 4
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.210723.001
DO  - 10.2991/ahsr.k.210723.001
ID  - Pratiwi2021
ER  -