Viability and Survival of Lactobacillus acidophilus Soygurt in the Gastric of Rat

DOI

10.2991/978-94-6463-060-2_16How to use a DOI?

Keywords

Lactobacillus acidophilus; Lactic acid; Soygurt

Abstract

Due to harsh environment that must be passed in digestive system, one of important characteristic of lactic acid bacteria is acid tolerance which leads an ability to survive in the gastric. In the current study, rat gastric was used to evaluate a viability and a survival of Lactobacillus acidophilus in soybean based soygurt. Preliminary in vitro study suggested that the growth of L. acidophilus was pH dependent. The soygurt containing 17.8 log10 CFU/mL of L. acidophilus was administered orally to the rats per day. After 7 days consecutive administration, the viability of L. acidophilus in gastric juice of rats was assessed. The protein profile and the presence of lactic acid in the gastric juice were analyzed. The result showed that 9.2 log10 CFU/mL of L. acidophilus was survived to pass in gastric. The pH of the gastric juice was more acidic in the presence of lactic acid after soygurt administration compared to control. The protein profile of juice contained specific protein with molecular mass about 100, 50, 37 kDa and small peptide which probably secreted as bacterial responses against gastric environment. These findings suggested that L. acidophilus in soygurt is viable and survived in gastric environment and this soygurt is qualified as an alternative probiotic for human, especially with lactose intolerance.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

TY  - CONF
AU  - Sayu Putu Yuni Paryati
AU  - Khomaini Hasan
AU  - Seno Wibiseno
PY  - 2022
DA  - 2022/12/16
TI  - Viability and Survival of Lactobacillus acidophilus Soygurt in the Gastric of Rat
BT  - Proceedings of The 13th Annual Scientific Conference of Medical Faculty, Universitas Jenderal Achmad Yani (ASCMF 2022)
PB  - Atlantis Press
SP  - 111
EP  - 118
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-060-2_16
DO  - 10.2991/978-94-6463-060-2_16
ID  - Paryati2022
ER  -