Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference

Overexpression of the Tryptophan Cluster in Corynebacterium Glutamicum

Authors
Hansong Yu, Xiaodi Liu, Jiahuan Liu,, Yuhua Wang, Yaohui Hu
Corresponding Author
Hansong Yu
Available Online June 2015.
DOI
10.2991/ap3er-15.2015.66How to use a DOI?
Keywords
Corynebacterium glutamicum; tryptophan operon; overexpression
Abstract

Extract the total DNA of HYHI, Trp cluster were cloned by PCR according to the DNA of HYHI, the length is 7100bp. Then the gene was connect to the expression vector pZ8-1, recombinant plasmid pZ8-1-Trp cluster was obtained. The plasmid was transferred to HYHI. The positive bacteria were induced by IPTG and determination by SDS-PAGE. The consequence show that the gene cluster is expression. And the same time, anthranilic acid synthase and tryptophan synthetase activity compared with the control increased 2.4 and 3.1 times, respectively

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference
Series
Advances in Engineering Research
Publication Date
June 2015
ISBN
978-94-62520-63-9
ISSN
2352-5401
DOI
10.2991/ap3er-15.2015.66How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hansong Yu
AU  - Xiaodi Liu
AU  - Jiahuan Liu,
AU  - Yuhua Wang
AU  - Yaohui Hu
PY  - 2015/06
DA  - 2015/06
TI  - Overexpression of the Tryptophan Cluster in Corynebacterium Glutamicum
BT  - Proceedings of the 2015 Asia-Pacific Energy Equipment Engineering Research Conference
PB  - Atlantis Press
SP  - 281
EP  - 284
SN  - 2352-5401
UR  - https://doi.org/10.2991/ap3er-15.2015.66
DO  - 10.2991/ap3er-15.2015.66
ID  - Yu2015/06
ER  -