Determination of Paeonol in Cynanchum paniculatum by high Performance Capillary Electrophoresis

DOI

10.2991/aeece-15.2015.144How to use a DOI?

Keywords

HPCE, Cynanchum paniculatum, paeonol

Abstract

This design uses High Performance Capillary Electrophoresis (HPCE) to determine the Paeonol’s content in Cynanchum paniculatum. Electrophoresis separation controlled conditions for uncoated fused silica capillary column (75 m×52 /60cm) injection height 7.5cm, and using a concentration of 40mmol/L borax solution as buffer. Using separate voltage 20KV to detect wavelength 270nm, temperature is 20°C, and the injection time control 10s. The Paeonol is linear relationship(r=0.998) within concentration of 3~400mg/L, and measuring 6 times its average recovery was 92.35%, RSD2.4%. After the contents were measured 7 times, Paeonol contain was 9.9mg/g RSD=6.6%. Conclusion for this design is that the method is simple, accurate and efficient with good reproducibility for the determination of paeonol in Cynanchum paniculatum determination.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Haixing Liu
AU  - Xiaodong Jiang
AU  - Xia Wang
AU  - Lintong Wang
AU  - Yunchen Zhang
PY  - 2015/09
DA  - 2015/09
TI  - Determination of Paeonol in Cynanchum paniculatum by high Performance Capillary Electrophoresis
BT  - Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering
PB  - Atlantis Press
SP  - 715
EP  - 718
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-15.2015.144
DO  - 10.2991/aeece-15.2015.144
ID  - Liu2015/09
ER  -