Artery Research

Volume 16, Issue C, December 2016, Pages 68 - 68

9.1 ULTRASOUND CHARACTERIZATION OF CARDIOVASCULAR ALTERATIONS IN YOUNG OB/OB MICE

Authors
Nicole Di Lascio1, Claudia Kusmic2, Francesco Stea2, Francesco Faita2
1Institute of Life Science, Scuola Superiore Sant’Anna, Pisa, Italy
2Institute of Clinical Physiology, National Research Council, Pisa, Italy
Available Online 24 November 2016.
DOI
10.1016/j.artres.2016.10.068How to use a DOI?
Abstract

Obesity is associated with diabetes and an increased cardiovascular risk. Leptin-deficient mice (ob/ob) are used as model of metabolic disease: they are characterized by obesity and insulin-resistance. Aim of this work is to identify early cardiovascular alterations in young ob/ob mice using micro-ultrasound imaging.

Sixteen wild-type (wt) and eleven ob/ob male mice (8 wo,C57BL6) were studied. B-mode and PW-Doppler images were acquired with a micro-ultrasonographic system (Vevo2100) for assessing cardiovascular biomarkers. Left ventricular mass (LVmass), cardiac output (CO), ejection fraction (EF), stroke volume (SV), fractional shortening (FS) and E/A ratio were measured. Mean diameter (Dm_abd and Dm_car), relative distension (relD_abd and relD_car) and pulse wave velocity (PWVabd and PWVcar) were obtained for both abdominal aorta and common carotid. As regards renal microcirculation, renal resistivity and pulsatility index (RI and PI) were assessed. The ratio between grey-levels related to liver and kidney (HR_ratio) was used as index of hepatic steatosis grade.

ob/ob mice had higher glycemia levels (ob/ob:296±42mg/dl, wt:149±23mg/dl, p<0.01) and higher weight (ob/ob: 44.2g, wt: 31.1g, p<0.01). relD abd values were lower for ob/ob mice than for wt ones (ob/ob:18.6±4.1%, wt:23.8±3.3%, p<0.01) the ob/ob group presented also higher PWV (ob/ob:2.11±0.69m/s, wt:1.73±0.43m/s, p<0.05), RI (ob/ob:0.71±0.05, wt:0.64±0.06m/s, p<0.01) and PI (ob/ob:1.15±0.17, wt:0.97±0.12m/s, p<0.01) values. As concerns hepatic steatosis, there was a difference in HR ratio evaluations (ob/ob:1.27±0.26, wt:0.79±0.17, p<0.01).!

Young ob/ob mice have a reduced abdominal aorta distension capability and a higher value of stiffness for this vessel. Moreover, starting from young age, parameters related to renal microcirculation and hepatic fat accumulation are altered.

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Journal
Artery Research
Volume-Issue
16 - C
Pages
68 - 68
Publication Date
2016/11/24
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2016.10.068How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - Nicole Di Lascio
AU  - Claudia Kusmic
AU  - Francesco Stea
AU  - Francesco Faita
PY  - 2016
DA  - 2016/11/24
TI  - 9.1 ULTRASOUND CHARACTERIZATION OF CARDIOVASCULAR ALTERATIONS IN YOUNG OB/OB MICE
JO  - Artery Research
SP  - 68
EP  - 68
VL  - 16
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2016.10.068
DO  - 10.1016/j.artres.2016.10.068
ID  - DiLascio2016
ER  -