P7.03 DIFFERENT EFFECT OF NEURONAL AND ENDOTHELIAL NOS INHIBITION ON HEART, CORONARY AND CAROTID ARTERY OF WISTAR RATS
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Long-term decrease of nitric oxide (NO) production evokes alterations in function and structure of cardiovascular system. We demonstrate the long-term effect of NO synthases inhibition with two different inhibitors on blood pressure (BP), heart and structure of coronary and carotid arteries. Four groups of ten weeks old Wistar rats were used: 1) control rats and rats receiving 2) NG-nitro-L-arginine methylester (L-NAME) (50 mg/kg/day in tap water), 3) 7-nitroindazole (7NI) (10 mg/kg/day in tap water), 4) the same doses of L-NAME (in tap water) and 7NI (in pellet diet). The experiment lasted 6 weeks. BP was measured by the plethysmographic method weekly. At the end of the experiment the animals were perfusion with a glutaraldehyde fixative (120 mmHg) and heart, coronary and carotid artery were excised and processed for electron microscopy.
| Wistar | L-NAME | 7NI | L-NAME+7NI | |
|---|---|---|---|---|
| BP (mmHg) | 112±2.20 | 164±2.10* | 118±1,90+ | 131±5,12*+# |
| H/B (mg/g) | 3,08±0,14 | 3,41±0,13* | 2,64±0,10*+ | 3,07±0,12+# |
| Coronary a. | ||||
| CSA (μm2) | 8860±800 | 18280±1320* | 6780±610*+ | 14250±2290*+# |
| Carotid a. | ||||
| CSA (μm2) | 69190±2460 | 109780±4040* | 54290±2200*+ | 88950±4340*+# |
Blood pressure (BP), heart/body weight (H/B), cross sectional area – intima+media (CSA).
P<0.01 vs. Wistar,
P<0.01 vs. L-NAME,
P<0.01 vs. 7NI
The study revealed, for the first time, that long-term inhibition of nNOS with 7NI, contrary to eNOS inhibition with nonspecific inhibitor L-NAME, evoked pressure independent cardiac hypotrophy and hypotrophy of the vessel wall. The data suggested that long-term nNOS inhibition likely iniciates a different chain of events leading to alterations in cardiovacular system then those after eNOS inhibition.
The study was supported by VEGA grant 2/0111/10, 2/0019/09.
Cite this article
TY - JOUR AU - F. Kristek AU - S. Cacanyiova AU - M. Gerova PY - 2010 DA - 2010/12/02 TI - P7.03 DIFFERENT EFFECT OF NEURONAL AND ENDOTHELIAL NOS INHIBITION ON HEART, CORONARY AND CAROTID ARTERY OF WISTAR RATS JO - Artery Research SP - 168 EP - 168 VL - 4 IS - 4 SN - 1876-4401 UR - https://doi.org/10.1016/j.artres.2010.10.080 DO - 10.1016/j.artres.2010.10.080 ID - Kristek2010 ER -