IL-17 producing CD4+CD45RO+ T-cells in atherosclerosis express GITR molecule

Atefe Ghamar Talepoor; Negar Behnamfar; Mohammad Javad Zibaeenezhad; Mehrnoosh Doroudchi

doi:10.1016/j.artres.2017.12.004

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Volume 21, Issue C, March 2018, Pages 20 - 28

IL-17 producing CD4+CD45RO+ T-cells in atherosclerosis express GITR molecule

Authors

Atefe Ghamar Talepoor^a^{, c}, Negar Behnamfar^a^{, c}, Mohammad Javad Zibaeenezhad^b, Mehrnoosh Doroudchi^a^{, *}

^aMemory T-cell Laboratory, Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

^bCardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

^c

These authors contributed equally in this manuscript.

^*Corresponding author. Memory T-cell Laboratory, Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, P.O. Box: 71345-3119, 71348-45794 Shiraz, Iran. Fax: +98 (71) 32351575. E-mail address: mdoroud@sums.ac.ir (M. Doroudchi).

Corresponding Author

Mehrnoosh Doroudchi

Received 29 October 2017, Revised 6 December 2017, Accepted 11 December 2017, Available Online 27 December 2017.

DOI: 10.1016/j.artres.2017.12.004 How to use a DOI?
Keywords: Atherosclerosis; Immunopathology; T cell; Inflammation
Abstract: Background: Atherosclerosis (AS) is a chronic inflammatory disease of vessel walls associated with infiltration of immune cells which their function is controlled by different co-stimulatory and co-inhibitory receptors. We investigated the expression of co-inhibitory molecules on the memory and effector T-cells in patients with Atherosclerosis.

Methods: Patients included 9 hypertensive, dyslipidemic, non-diabetic, non-smoker individuals with the diagnosis of coronary artery disease and controls were 8 normotensive, normolipemic, non-diabetic, non-smoker individuals with normal coronary angiography/insignificant coronary artery disease. PBMCs were separated from the blood and memory T-cell subsets as well as the expression of Glucocorticoid-induced tumor necrosis factor receptor (GITR), Programmed Death-1 (PD-1), IL-17A and IFN-γ were quantified by flowcytometry.

Results: CD4+CD45RO+ memory T-cells and CD4+CD45RO− effector T-cells in patients expressed the highest level of GITR molecule. The IL-17 producing memory CD4+CD45RO+ T-cells were enriched in GITR molecule in the patients group (P = 0.03). The increased population of GITR+effector CD4+CD45RO− T-cells in patients, however, did not produce IL-17 (P = 0.03). PD-1 expression on memory T-cells of the patients was higher than the controls and was concomitant with the lack of IFN-γ expression (P = 0.05). IFN-γ production by effector T-cells was only seen in the PD-1− population in both groups.

Conclusions: We provide data on the expression of GITR molecule on IL-17 producing memory T-cells in patients with CAD. A population of memory T-cells, which expressed PD-1 and were not producing IFN-γ, also increased in patients’ blood. These data suggest the modified phenotype/function of T-cell subsets in the atherosclerotic inflammation.
Open Access: This is an open access article distributed under the CC BY-NC license.

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Journal: Artery Research
Volume-Issue: 21 - C
Pages: 20 - 28
Publication Date: 2017/12/27
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2017.12.004 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

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ris enw bib

TY  - JOUR
AU  - Atefe Ghamar Talepoor
AU  - Negar Behnamfar
AU  - Mohammad Javad Zibaeenezhad
AU  - Mehrnoosh Doroudchi
PY  - 2017
DA  - 2017/12/27
TI  - IL-17 producing CD4+CD45RO+ T-cells in atherosclerosis express GITR molecule
JO  - Artery Research
SP  - 20
EP  - 28
VL  - 21
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.12.004
DO  - 10.1016/j.artres.2017.12.004
ID  - Talepoor2017
ER  -

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