Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017)

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94 articles

The Development Status and Suggestions of Chinese Biological Medicine

Jin-guo WANG, Na WANG
Biological medicine is listed on the country's important development strategy planning in the United States, the European Union, Japan and other major developed countries and some developing countries. Through analysis of Chinese current situation of the development of biological medicine and compared...

Research on Concanavalin A Crystallization in a Flow-Free Droplet-Based Device

Guan-ru DU, Wen-jun ZHANG, Ding-kun JI, Feng-juan CHEN, Di YIN, Hong-bo ZHANG, Rui-xue YIN, Xiao-peng HE, Shih-Mo YANG
Protein crystallography is a very critical process that limits the development of pharmaceutical engineering. Microfluidic technology has provided an opportunity for protein crystallization. In this paper, a flow-free droplet-based device is presented for producing highly controlled crystals and fabrication...

Cerebral Microbleed Detection by Wavelet Entropy and Naive Bayes Classifier

Hai-nan WANG, Beatrice Gagnon
(Aim) Current cerebral microbleed detection methods are too complicated, and difficult to train. (Method) We enrolled 10 subjects diagnosed as cerebral microbleed.Our method combined wavelet entropy and naive Bayes classifier. (Results) The simulation results over 10 times of 10-fold cross validation...

Enhancement of Viability for Staphylococcus Aureus During Freeze-Drying Using the Response Surface Methodology

Hai-li YANG, Jin-xia LI, Dong-guang XIAO, Hai-bo DU, Bo LIU, Yi LIU, Hai-rong HU
Response surface methodology (RSM) was used to optimize a protective agent for enhancing the cell viability of Staphylococcus aureus during freeze-drying. Using a previous Plackett-Burman design, it was found that sodium glutamate, trehalose, L-cysteine hydrochloride and bovine serum albumin were the...

Liver Vessel Segmentation Algorithm Based on Fuzzy Enhance and Hessian Matrix

Yue-jing QIAN
This paper proposes a liver vessel segmentation algorithm based on fuzzy enhancement and Hessian matrix. First, a method with two steps based on the theory of fuzzy enhancement is proposed to enhance the liver image. The first enhancement step is to make the local changes in the image more obvious on...

Research on Intracranial Pressure Changes of Craniocerebral Injury Caused by Cudgels

Hong-fa CHANG, Zhen-jiang LIU, Peng XIA, Yong-min YU, Dai-qin TAO, Hong-wei LI
Objective To research intracranial pressure change rules of craniocerebral injury through experimentation on animals. Method According to the difference of the hit parts, the animals are divided into three groups to collect the intracranial pressure changes through data collection system andpressure...

The Neural Mechanism of Acupuncture Analgesia

Qiu-ling XU, Tao LIU
The acupuncture signal in the uplink conduction, one side through the spinal cord segmental relationship affect the adjacent segments dominated by the skin, visceral activity and adjacent segments of the pain in the introduction; more often up to reach the brain stem, Forebrain and other parts, through...

Research Progress of Plant Calcium Nutrition and Calcium / Cold Signal Interaction

Qing-wen SHI, Qiao-bo SONG, Hui-xin WANG, Chun-ming BAI, Di WU, Qi-ping DONG, Xin CHENG, Xiao-ri HAN, Yi-fei LIU
Calcium, as the second messenger inside plants, is extensively involved in plant response and transduction of various stress signals. Plants shall generate calcium signals of specificity under stress, and this shall give a rise to a series of intracellular physiological and biochemical responses. Calcium...

Injured Human Kidney Proximal Tubular Epithelial Cells Modulate Nucleation and Growth of Calcium Oxalate Crystals

Da GUO, Jian-min WANG, Jian-ming OUYANG
Purpose: The oxidative damage of hydrogen peroxide (H2O2) on human kidney proximal tubular epithelial cell (HKC) and the nucleation and growth of calcium oxalate (CaOx) crystals modulated by normal and injured cells were investigated. Methods: The method of CCK-8 was used to detect the proliferating...

Identifying the Sulfate Ion Binding Residues in Proteins

Shao-bo LI, Xiu-zhen HU, Li-xia SUN, Xiao-jin ZHANG
Many proteins function execution depends on the process of protein and ligand interact with each other. The identification of ligand binding residues is important for the research of the protein function. The 4442 protein chains with <25% sequence identity and resolution <3.0  were analyzed using Ligand...

Correlation between Analgesic Effect of Electroacupuncture "ST36" - "GB34" and DNA Methyltransferase on Sciatica Rats Dnmt3a

Qiu-ling XU, Tao LIU
To observationthe relationship between spinal inhibition of DNA methylation in electroacupuncture (EA) analgesia, and spinal cord Dnmt3amRNA expression. EA and inhibitors of 5-AZA Dnmt can improve CCI animals hyperalgesia state and decreased Dnmt3amRNA expression. DNA methylation is closely related to...

Soft Renal Calyx Calculi under Ureter Mirror Treatment

Fan-ping MENG, Yan HE, Di ZOU, Ji GAO
Objective: to study the electronic soft ureter mirror joint qin laser lithotripsy in the treatment of renal calyx calculi under safety and effectiveness.Methods: our hospital in March 2016 - March 2017 electronic soft ureter mirror qin laser lithotripsy under 40 patients under the treatment of renal...

Direct Electrochemistry of Glucose Oxidase Immobilized on a Hydroxyl Fullerenes- Tio2 Nanocomposite Modified Glassy Carbon Electrode

Di LI, Xiao-Yu ZHANG, Bao-Lin XIAO, Jun HONG, Moosavi-Movahedi Ali Akbar
By immobilizing glucose oxidase (GOD) on a nanocomposite containing hydroxyl fullerenes (C60-OH) and TiO2 nanoparticles, a new type biosensor was prepared for glucose detection. The cyclic voltammograms (CVs) of GOD on the nanocomposite modified glassy carbon electrode (GCE) was quasi-reversible. The...

Cloning, Expression and Enzymatic Characterization of Chitin Deacetylase 4 from Hyphantria Cunea (Drury)

Xiao-ping YAN, Dan ZHAO, Ya-kun ZHANG, Wei GUO, Kun-li ZHAO, Wei WANG, Yu-jie GAO
A novel chitin deacetylase (CDA), HcCDA4, was identified from the American white moth, Hyphantria cunea. The full-length cDNA sequence of HcCDA4 was identified,and the cDNA is 1494bp in length. The HcCDA4 was shown as a 56 kDa protein in E. coli BL21 by SDS-PAGE analysis and the specific antibodies reacting...

Network Pharmacology of JinChaiKangBingDu Capsule Against Influenza A (H1N1) Virus Infection

Zhi-de CHENG, Xiao-jing WANG, Peng DENG, Jing ZHAO, Gao CHEN
Pharmacological mechanisms of Chinese herbal prescription are complicate since it contains heterogeneous components. These mechanisms are hardly understood by traditional methodology in the mass. Here we use network pharmacology approaches to explore mechanisms of JinChaiKangBingDu Capsule (JCKBD) against...

Ultrastructure and Self-Cleaning Function of Moth (Notodontidae) and Butterfly (Lycaenidae) Wings

Yan FANG, Gang SUN, Jing-shi YIN, Wan-xing WANG, Yu-qian WANG
The microstructure, hydrophobicity, adhesion and chemical composition of the butterfly and moth wing surfaces were investigated by a scanning electron microscope (SEM), a contact angle meter, and a Fourier transform infrared spectrometer (FT-IR). Using ground calcium carbonate (heavy CaCO3) as contaminating...

Targeted Therapies for Alzheimer's Disease: An Overview

Yong SUN, Ying-ju XIU, Ying XING
Dementia is the most common onset of Alzheimer's disease (AD). The main neuropathological characteristics of AD are extracellular amyloid plaques and intracellular accumulation of hyperphosphorylated Tau protein. Because of the complexity of the pathogenesis, the most treatment options are not effective...

Basic Research on Real-time Evaluating of Thermal Damage of Tissues by Visible-Near Infrared Spectra

Wei-wei JIA, Li-juan DAI, Xi WANG
The feasibility of evaluation thermal damage in real time by Visible-Near Infrared (V-NIR) spectroscopy is investigated. Thermal damage are produced in fresh porcine livers in vitro by laser (808nm, 5W). The V-NIR spectra (330-1100 nm) at distances of 4, 8 and 12 mm from the center of the damage are...

Antibiotics and ELISA as a Hyphenated Technique for Screening High Production Cell Lines from Transfected HEK293 Cells for the Production of Recombinant Follicle Stimulating Hormone

Jian-wen HU, Meng-meng LIU, Ji-zhong HAN, Bin ZENG
Recombinant follicle stimulating hormone(rFSH), which play an important role in assisted reproductive technologies. However, the availability of a suitable system for the screening high production cell lines from transfected mammal cells for the production of rFSH is limited. The purpose of this study...

Extracellular Expression and Catalytic Bioactivity of Optimized Human Aromatase (CYP19) in Pichia Pastoris

Yan-ying HU, Yong-hui ZHONG, Jing ZHEN, Hui-tu ZHANG, Shi-ru JIA, Fu-ping LU, Yu-jie DAI, Xiu-li ZHANG
The fragment encoding aromatase (CYP19) with 45 N-terminal amino acids cut-off was cloned into pPIC9K vector and transformed to the yeast Pichia pastoris. The multi-copy recombinant strain was selected for the production of recombinant aromatase. The catalytic bioactivity of secreted enzyme was further...

Analysis of Animal Models of Oral Ulcers Based on the Characteristics of Clinical Symptoms of Chinese and Western Medicine

Huan LI, Ming BAI, Yan-yan MIAO, Ming-san MIAO
Objective: To analyze the methods and characteristics of animal model of oral ulcer and the characteristics of Chinese and Western medicine oral ulcer disease anastomosis. Methods: To summarize the characteristics of animal models of oral ulcers, modeling methods, analysis of oral ulcer animal model...

Correction of Sleep Staging Based on Sleep Spindle Detection

Xue-mei JIN, Jun-zhong ZOU, Jian ZHANG
It has been demonstrated that sleep has a great relationship with humans' health and activities. Sleep staging is an important method to analysis sleep. Lots of methods have been adopted for automatic sleep staging. Some of the results are good and some of them are poor. For the poor, the sleep spindle...

MEG Weak Source Imaging Methods Based on Functional Connectivity

Ting WU, Yuan CUI, Zhi-guo ZHANG, Jun-peng ZHANG, Tian-zi JIANG
Beam former, as a class of Magnetoencephalography (MEG) source imaging methods, is widely used. However, it has difficulty in locating weak electromagnetic sources within brain. To solve this problem, this paper attempts to develop a method for MEG weak Source Imaging. Firstly classical beam former is...

Effects of Jinjiang Granules on Immunologic Function of Immunosuppressive Mice Model Induced by Cyclophosphamide

Ming-san MIAO, Li-hua CAO, Xue-xia ZHANG
Objective: To explore the effects of jinjiang granules on immunologic function and to provide evidence for jinjiang granules as the application of clinical medicine in the initial stage of cold, through the observation of the effects of jinjiang granules on immunologic function of low immune mouse model...

Study on the Infrared Spectra of Superoxide Dismutase with the Different Treatment Intensity of Electric Field

Siqin-gaowa BAO, Yan-ru WANG, Zhi-chao CHEN, Zhao WEI, Zhan-xin MA
With the different treatment intensity of electric field, we analyze the influence of electric field on infrared spectra of superoxide dismutase by using infrared spectra method. The results show that, superoxide dismutase (SOD) is treated with different electric field intensity, the absorption peak...

Qualities and Aroma of Watermelon Juice during Storage at 4 øC

Yu-bin WANG, Yue MA, Xiao-yan ZHAO, Chao ZHANG
The watermelon juice was pasteurized by the ultra-high temperature treatment, and stored at 4 øC for 2 weeks. The ultra-high temperature reduced the initial microbial counts of the watermelon juice. The aroma of the watermelon juice was reduced during the storage at 4 øC for 2 weeks. The typical volatiles...

Identification of a Bacillus Thuringiensis Cry8Ea3 to Xin-Binding Alkaline Phosphatase from Holotrichiaparallela

Wei WANG, Dan ZHAO, Wei GUO, Xiao-ping YAN, Ya-kun ZHANG, Kun-li ZHAO, Yu-jie GAO, Xiao-yun WANG
In order to further study the Insecticidal Mechanism of Bt protein against Holotricia parallela. In the study, According to the results of midgut transcriptome sequencing and RACE-PCR, the full-length hpalp gene was cloned. Sequencing analysis showed that the open reading frame of hpalp (GenBank accession...

Analysis on the Characteristics of Flower Traditional Chinese Medicine for Preventing and Treating Cerebral Ischemia

Li-ling XIANG, Ming-san MIAO, Ya-juan WEN, Jing-yi QIAO
Objectives: To investigate the characteristics and mechanism of flower traditional Chinese medicine for preventing and treating cerebral ischemia. methods: To summarize active components of flower traditional Chinese medicine on cerebral ischemia model and the characteristics of prevention and treatment...

Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells

Li-shan HUANG, Da GUO, Jian-ming OUYANG
Purpose: To study the injury difference of the un-aggregated and aggregated calcium oxalate monohydrate (COM) on human kidney proximal tubular epithelial (HKC) cells, and to discuss the molecular and cellular mechanism of kidney stone formation. Methods: The cell injure by crystals was evaluated by measuring...

Study on Terpenoid Compounds of Different Tissues of Camphor Tree

Ying-ying YUE, Yan FU, Jie CHANG
Based on the current research value of biomass and terpenoids, in this paper, the terpenoids contented in the different organizational of the camphor tree were analyzed by SDE extract method and GC-MS analysis method. The results showed that the contents of terpenoids contained in camphor leaves were...

Characterization of Microbial Community Composition in the Fermentation Starter (Daqu) Reveals a Distinct Dominant Species Combination Potentially Contributing to the Flavor of Chinese Gujingtribute Liquor

Hui-min ZHANG, Hong-kui HE, Run-jie CAO, Lei LIU, Zhi-hui TANG, An-jun LI, Zhi-zhou ZHANG
In this study the microbial composition in the starter (Daqu) of Chinese Gujing Tribute liquor was determined by employing near full length ribosome gene library sequencing approach. Gujing Tribute Daqu has a distinct microbial community structure by comparison with several other important types of Chinese...

Anti-Proliferative Activity of Benzamide Analogs in A549 Tumor Cells in Vitro

Li-wen SHEN, Yan-ling WU, Min CHEN, Yoshimasa Tanaka, Wen ZHANG
Objective: To determine the antitumor activity of five benzamide derivatives with similar structure in A549 tumor cells. Methods: Cytotoxicity of the compounds in A549 tumor cells were monitored through an xCELLigence Real-Time Cell Analysis System. Results: Four benzamide analogs inhibited the growth...

Aqueous Two-Phase System Combined with Ultrasound for the Extraction Of Polysaccharide from Hawthorn (Crataeguspinnatifida) Leaves

Ri-li HAO, Juan-juan MA, Kun LI, Chuan-he ZHU, Xiao-cun ZHANG
Aqueous two-phase system (ATPS) combined with ultrasonic technology was employed to extract polysaccharide from hawthorn leaves in this study. The partition behaviors in different ATPS were investigated first, polyethylene glycol (PEG) 11.5% and (NH4)2SO4 14% were selected as the optimum solvent for...

Molecular Actions of Marine Bioactive Compound Fucoidan

Xue-lian WANG, Yan ZHAO
Fucoidan is a complex sulfated polysaccharide mainly found in brown seaweed. It has been used in a wide range of applicationsacross different industries such as food additives, dietary supplements and pharmaceuticals. Studies have shown that fucoidan has numerous biological activities including anti-cancer,...

Effect of Mental Fatigue on Error Monitoring

Feng MA, Yi XIAO, Gui CAI, Feng-gang XU, Shan-guang CHEN
Performance monitoring tasks generate response-locked event-related potentials (ERPs), generated from anterior cingulate cortex (ACC) region of the medial prefrontal cortex. However, the quantitative relationship between mental fatigue and error monitoring has not been closely investigated, which is...

Microrna -145 Expression Inhibits Invasion and Metastasis of Cervical Cancer Cells

Shun-qing ZHOU, Ning LIU, Yang LIN
[Purposes] To investigate the expression of microRNA-145 on invasion and metastasis of cervical cancer cells. [Methods] Analysis of the relationship between high expression of microRNA-145 on invasion and metastasis of cervical cancer cells by Transwell invasion assay and Wound scratch assay. [Results]...

Development of a Novel Multi-Functional Rehabilitation Robot

Cheng-dong WEI, Quan-quan LIU, Li-hong DUAN, Tong-yang SUN, Meng LI, Zhan ZENG, Chun-bao WANG, Qing SHI, Zhuo-hua LIN, Ya-jing SHEN, Wan-feng SHANG, Zheng-zhi WU
With the development of aging society, the number of hemiplegia patients grows rapidly and over 75% of survivors suffering hemiplegia need rehabilitation training. Traditional training methods need one or more physical therapists to server one patient at the same time. In order to reduce the workload...

Astaxanthin in Disease Prevention and Treatment

Jun WANG, Yan ZHAO
Astaxanthin is a carotenoid with strong antioxidant activity. The function of astaxanthin has been associated with a number of health-promoting benefits. This paper discusses the beneficial effects of astaxanthinin the prevention and treatment of human diseases such as cardiovascular diseases, cancer,...

Analysis of Vitiligo Model Based on Clinical Features of Traditional Chinese Medicine and Western Medicine

Xiao-li YAN, Li-hua CAO, Ming-san MIAO
Objective: Analysis common animal models and vitiligo clinical symptoms anastomotic situation, put forward recommendations of improving the model. Methods: By summarizing the characteristics and modeling methods of vitiligo model and according to the clinical diagnostic criteria of vitiligo on TCM and...

Amide Resonance Structure Detected by NMR to Predict Hydroxyl Unit in Protein

Yan JI, Xiao-liang YANG, Jun-ming TANG
Amide tautomer imidic acid were exit, which proved by NMR spectra characterization. The protein BSA was test by 1H NMR in different pH values solutions, to show the different amide/imidic acid structures exit in BSA. The amide/imidic-acid unit with H proton communication might be the biological protein...

Use Text Mining and Complex Networks to Analyze TCM Syndromes

Xing ZHAI, Ping WANG, You-liang HUANG
Objective: To make intrinsic biological distinctions between qi deficiency pattern and qi stagnation pattern with genes related to NEI. Methods: Establish a data dictionary of NEI-related genes and a keyword lexicon of qi deficiency pattern and qi stagnation pattern. Then retrieve relevant literature...

New Generation Cardiac SPECT Systems

Jin-hua SHENG, Jian-hai ZHANG
The cardiac SPECT is a good study because of the large clinical demand and the need for improved image quality. SPECT imaging usually suffers from poor spatial resolution and high statistical noise due to patient radiation safety concerns. Scientists proposed some high count sensitivity cardiac SPECT...

Inference of Pure Titanium Micro-arc Oxidation Process on Microstructure of Calcium Phosphate Membrane Layer

Yu-lei LI, Jin-jun TANG, Yue-lai DAI, Li-jun ZHANG, Xiao-yu JIN
In this paper, the growth law of the phosphate and calcium layer in different voltage process was studied. The surface morphology of the film was observed by SEM, and then the distribution of phosphorus and calcium in the film was analyzed. The results show that the higher the voltage, the more uniform...

A Study on the Local Electrochemical Processes Inside Cavities

X. T. SUN, C. Y. ZHANG, X. S. CHEN
In this paper, a set of experiments scanning real potential variations inside cavities under current control were employed to characterize local electrochemical information. The limiting diffusion current and the critical currents sustaining the supersaturation of dissolved gas and completed oxide film-formation...