Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India

Rakesh Yadav; Pankaj Vaidya; Joseph L Mathew; Sanjay Verma; Rajiv Khaneja; Priyanka Agarwal; Pankaj Kumar; Meenu Singh; Sunil Sethi

doi:10.2991/jegh.k.191215.002

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Volume 10, Issue 2, June 2020, Pages 153 - 156

Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India

Authors

Rakesh Yadav¹^{, #}, Pankaj Vaidya²^{, #}, Joseph L Mathew², Sanjay Verma², Rajiv Khaneja³, Priyanka Agarwal⁴, Pankaj Kumar⁵, Meenu Singh², Sunil Sethi¹^{, *}

¹Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India

²Pediatric Medicine, Advanced Pediatric Center, Postgraduate Institute of Medical Education and Research, Chandigarh, India

³State TB Cell, Sector 34, Chandigarh, India

⁴WHO Country Office for India, New Delhi, India

⁵Department of Paediatrics, Govt Medical College, Chandigarh, India

^#

First two authors are joint first author.

^*Corresponding author. Email: sunilsethi10@hotmail.com

Corresponding Author

Sunil Sethi

Received 7 June 2019, Accepted 7 December 2019, Available Online 10 January 2020.

DOI: 10.2991/jegh.k.191215.002 How to use a DOI?
Keywords: Xpert MTB/RIF; pediatric tuberculosis; diagnosis; India
Abstract: Tuberculosis (TB) diagnosis in children still remains a challenge in developing countries. We analyze the performance of Xpert MTB/RIF assay for the diagnosis of pediatric TB under programmatic conditions. We retrospectively analyzed the performance of Xpert MTB/RIF assay from February 2016 to March 2018. A total 2678 samples from TB suspects below 14 years were received in the laboratory and were frontline tested by Xpert MTB/RIF assay according to the manufacturer’s instructions. If sample was sufficient, the smear microscopy and culture were performed as per standard World Health Organization’s guidelines. The smears and cultures were performed in 2178 and 588 samples, respectively. Among 2678 samples, 68 were rejected, Xpert MTB/RIF assay was positive in 357/2610 (13.6%) cases, while the smear was positive in 81/2178 (3.3%) cases. The sensitivity of smear and Xpert MTB/RIF when compared with culture was 24.6% (14.1–37.8%) and 81% (68.6–90.1%), respectively. The diagnostic accuracy of Xpert MTB/RIF and smear was 97.1% and 92.2%, respectively. Thirty samples (8.5%) were detected as rifampicin resistance by Xpert MTB/RIF assay. The Xpert MTB/RIF increased the detection rate up to fourfold when compared with smear microscopy. Xpert MTB/RIF assay is the most rapid, sensitive, and specific method for microbiological confirmation and rifampicin resistance detection in pediatric tuberculosis.
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal: Journal of Epidemiology and Global Health
Volume-Issue: 10 - 2
Pages: 153 - 156
Publication Date: 2020/01/10
ISSN (Online): 2210-6014
ISSN (Print): 2210-6006
DOI: 10.2991/jegh.k.191215.002 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - Rakesh Yadav
AU  - Pankaj Vaidya
AU  - Joseph L Mathew
AU  - Sanjay Verma
AU  - Rajiv Khaneja
AU  - Priyanka Agarwal
AU  - Pankaj Kumar
AU  - Meenu Singh
AU  - Sunil Sethi
PY  - 2020
DA  - 2020/01/10
TI  - Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India
JO  - Journal of Epidemiology and Global Health
SP  - 153
EP  - 156
VL  - 10
IS  - 2
SN  - 2210-6014
UR  - https://doi.org/10.2991/jegh.k.191215.002
DO  - 10.2991/jegh.k.191215.002
ID  - Yadav2020
ER  -

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